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Alt-R™ CRISPR-Cas9 Library design Tool

Easy online design & ordering for CRISPR screening

Streamline CRISPR screening with fast, online custom gRNA design and ordering. Upload targets directly, view plate maps, save designs, and get high-quality, modified gRNAs with fast delivery—all from your personalized dashboard.

Skip the consultations and emails—design and order directly

Advantages of Alt-R CRISPR-Cas9 libraries

  • Predesigned gene families available: Druggable genome, drug targets, transcription factors and more
  • Customize plate layout, gRNA pooling, and normalization for your experiment
  • Optional integration with rhAmpSeq analysis of on– and off-target editing confirmation
  • Visualized plate maps and easy editing before ordering

DESIGNING AND ORDERING CRISPR-CAS9 LIBRARIES JUST GOT EASIER

Tool Icon
Easy online design tool
Time Icon
Order in minutes
On/Off Target Icon
Integrated on-and-off target analysis
Flexibility Icon
Flexible and customizable
Features Benefits
Option to select from predesigned gene families No need to identify the genes of interest
Completed library designs are auto-saved in your dashboard and can be downloaded Always have access to your designs even when logging off
Easy options to customize pooling strategy, plate layout, normalization and formulation Customize your library and integrate into your workflow
Clear visual of your individual plate maps and their wells Easier to confirm that everything is correct and make final adjustments prior to ordering
Option to integrate rhAmpSeq™ design to your library design, synchronized to your library plate layout Reduces hurdles for on- and off-target analysis after editing

Ready to get started?

Sign-in or create an IDT account to start using the Alt-R CRISPR-Cas9 Library design Tool today!

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Tailored to your research

Using the Alt-R CRISPR Libraries design & ordering tool

  • Design input 0:08
  • Design validation: 1:50
  • Design summary & dashboard navigation: 2:30
  • Ordering overview: 4:25
  • Pooling & plate configuration: 5:04
  • Format options: 6:25

Alt-R CRISPR-Cas9 Libraries Design & Ordering Tool Tutorial

This video will guide you through the full process of designing and ordering your custom library. It walks you through many features including the dashboard, custom plate configurator, and the option to integrate rhAmpSeq NGS editing analysis downstream.