TruGrade DNA Oligos
With rapidly increasing throughput of sequencing platforms, sample multiplexing has become a common solution to economically address new sequencing capacities. However, pooling indexed samples into one sequencing run comes with the risk of sample misidentification. While this can be attributed to many experimental factors, crosstalk during oligo synthesis is a major contributor. TruGrade DNA oligos are manufactured using an exclusive production process that reduces the risk of oligonucleotide crosstalk and thereby minimizing the risk of barcode misalignment during multiplexing NGS applications.
The TruGrade synthesis and processing service is available for HPLC-purified DNA oligos and Ultramer™ DNA oligos. They are available in plates and tubes and can be formulated to your specifications. TruGrade DNA oligos can be used in a variety of applications including, but not limited to:
- Library preparation that incorporates barcoded adapters for multiplexing
- PCR using barcoded fusion primers for multiplex amplicon sequencing
- Other molecular biology applications that are sensitive to oligo crosstalk